Streaking Bacterial Stocks
Long-Term Storage of Bacterial
Strains
Toothpick Plasmid Assay
Transfer of DNA from Bacterial Colonies to
Nylon Membrane or Nitrocellulose
Alkaline Lysis Minipreps of Plasmid or
Cosmid DNA
Large-scale Plasmid Preparation
Recovery and Purification of Recombinant
Cosmid DNA Using a CsCl2 Gradient
Preparation of Miniprep Cosmid DNA from 50
ml Cultures
Preparation of Competent Cells
Transformation of Plasmids/Cosmids into E.
Coli
Ligations in Low Melting Temperature
Agarose
Plasmid Subcloning
Bacterial Media, Solutions, and
Stocks