COGENE Targets

Carnegie Stages Targeted

These tables include 46 biologically important developing craniofacial regions from different temporal stages and how COGENE will use them. For some explanations of the various Carnegie stages and developmental events in early embryogenesis follow these links: Carnegie stages (source: U. Mich), Carnegie stages (source: UNSW), all stages (source: UNSW), movies (source: UNSW).

Table key (at page bottom)

Stage A 3 weeks
Tissue	Proposed activity
whole embryo	C
cranial half of embryo	SAGE
(exclusion of caudal half), prior to anterior neuropore closure
Stage B 4-5 weeks
Tissue	Proposed activity
1st pharyngeal arch	C, S3, SAGE
2nd pharyngeal arch
3rd and 4th pharyngeal arch (combined if technically required)
combined rhombomeres 1-3, 3-5, and 5-8	C, SAGE
frontonasal prominence
maxillary prominence
mandibular prominence
Stage C 6-7 weeks
Tissue	Proposed activity
medial and lateral nasal processes (combined if technically required)
primary palate (combined with maxilla if technically required)
palatine shelves (combined with maxilla if technically required)
anterior 2/3 and posterior 1/3 tongue (combined if technically required)	C
maxilla	C, S1, SAGE
early mandible	C, S3, SAGE
calotte, membrane bone
chondrocranium, cartilaginous model
Stage D 7-8 weeks
Tissue	Proposed activity
formative nose, lateral and intermaxillary processes
primary palate, palatine shelves (pooled for SAGE)	SAGE
anterior 2/3 tongue posterior 1/3 tongue
maxilla	C, S1
dental lamina, developing teeth	C, S2, SAGE
mandible
calotte, membrane bone
basicranium	SAGE
Stage E 10 weeks
Tissue	Proposed activity
nose
palate
anterior 2/3 tongue
posterior 1/3 tongue
maxilla
tooth buds, individual
later mandible
salivary gland
calotte (dissecting leading edge of the bones at site of formative coronal and sagittal sutures if technically possible)
basicranium
Stage F >22 weeks and <3 months
Tissue	Proposed activity
anterior 2/3 tongue
posterior 1/3 tongue
maxilla	C, S1
tooth buds, individual	C, S2
mandible	C, S3
temporomandibular joint (glenoid fossa of parietal, condyle and meniscus)
salivary gland	C, SAGE
cranial coronal sutures, cranial sagittal sutures (pooled for SAGE)	SAGE

Table key.
Tissue Proposed:
    A stage from which we propose to make cDNA libraries C
    Stages that will be subtracted against each other
    (e.g. maxilla at stages C, D, and F) S1 through S8
    Stages targeted for SAGE analysis SAGE

Table key.
Tissue	Proposed:
A stage from which we propose to make cDNA libraries	C
Stages that will be subtracted against each other (e.g. maxilla at stages C, D, and F)	S1 through S8
Stages targeted for SAGE analysis	SAGE

last updated Sept. 14, 2000